IMPORTANT TERMS IN AUTOCLAVE
D – Value – This is the time estimated to kill 90% of Bio Load
F0 – Value – This is product specific sterilization time
APPROACH – Bio Burden or Over kill approach
TESTS – Vacuum Test (- 0.8 Bar ) hold for 10 Mins, while leak rate NMT 1.3 mbar / min
Air removal (Bow Dick Test)
Heat Distribution Test
Heat Penetration Test
DWELL TIME – Equilibrium Time + Sterilization Time
BI- Used for Qualification – Generally BI used are Bacillus Stero Thermophilus
PHASES OF STERILIZATION CYCLE –
CONDITIONING – Chamber Air replacement with steam + Heating
EXPOSURE – Actual time when Sterilization happens
DRYING – Vacuum pulses to remove moisture